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Identification of regulators for Ryanodine 2 Enhancer (Ryr2)
Objectives
In the summer of 2024, I worked with Dr. Nathan VanDusen on identifying regulators of ryanodine gene enhancer
Research Objectives:
•Understand which transcription factors play a role in the regulation of the Ryanodine 2 Enhancer
•Further understanding of the mechanism of calcium regulation in cardiomyocytes.
Date
Summer 2024
Identification of Regulators for Ryanodine 2 Enhancer
Strategy Practiced:
Create a plasmid with the removal of Streptavidin-Binding Peptide (SBP)-Tag from the plasmid
Insertion of the Hsp 68 promoter
Insertion of a library of guide RNAs for the formation of a genetic screen
Techniques practiced:
Column purification
Gel extraction
Gel purification
Whole plasmid sequencing
Sagner sequencing
Phusion reaction for removal of plasmid overhangs
Ligation protocol
Colony PCR
